Intracellular biotransformation and disposal mechanisms of magnetosomes in macrophages and cancer cells

Gandarias, L.; G. Gubieda, A.; Gorni, G.; Mathon, O.; Olivi, L.; Abad-Diaz-de-Cerio, A.; Fdez-Gubieda, M. L.; Muela, A.; Garcia-Prieto, A.

doi:10.1101/2023.03.15.532722

bioRxiv · 10.1101/2023.03.15.532722

Intracellular biotransformation and disposal mechanisms of magnetosomes in macrophages and cancer cells

Gandarias, L.·G. Gubieda, A.·Gorni, G.·Mathon, O.·Olivi, L.·Abad-Diaz-de-Cerio, A.·Fdez-Gubieda, M. L.·Muela, A.·Garcia-Prieto, A.

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Abstract

Magnetosomes are magnetite nanoparticles biosynthesized by magnetotactic bacteria. Given their potential clinical applications for the diagnosis and treatment of cancer, it is essential to understand what becomes of them once they are within the body. With this aim, here we have followed the intracellular long-term fate of magnetosomes in two cell types: cancer cells (A549 cell line), because they are the actual target for the therapeutic activity of the magnetosomes, and macrophages (RAW 264.7 cell line), because of their role at capturing foreign agents. We show that cells dispose of magnetosomes using three mechanisms: splitting them into daughter cells, excreting them to the surrounding environment, and degrading them yielding less or non-magnetic iron products. A deeper insight into the degradation mechanisms by means of time-resolved X-ray absorption near-edge structure (XANES) spectroscopy has allowed us to follow the intracellular biotransformation of magnetosomes by identifying and quantifying the iron species occurring during the process. In both cell types there is a first oxidation of magnetite to maghemite and then, earlier in macrophages than in cancer cells, ferrihydrite starts to appear. Given that ferrihydrite is the iron mineral phase stored in the cores of ferritin proteins, this suggests that cells use the iron released from the degradation of magnetosomes to load ferritin. Comparison of both cellular types evidences that macrophages are more efficient at disposing of magnetosomes than cancer cells, attributed to their role in degrading external debris and in iron homeostasis. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=113 SRC="FIGDIR/small/532722v3_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@1b0d41dorg.highwire.dtl.DTLVardef@35ebf9org.highwire.dtl.DTLVardef@172a97org.highwire.dtl.DTLVardef@ed58ff_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Gandarias, L., G. Gubieda, A., Gorni, G., Mathon, O., Olivi, L., Abad-Diaz-de-Cerio, A., Fdez-Gubieda, M. L., Muela, A., Garcia-Prieto, A.. 2023-03-15. Intracellular biotransformation and disposal mechanisms of magnetosomes in macrophages and cancer cells. https://doi.org/10.1101/2023.03.15.532722

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