Species-specific stomatal ABA responses in juvenile ferns grown from spores

Abstract

Adjustable stomatal pores in leaves control the balance between CO2 entry for photosynthesis and water loss via transpiration. The drought and low humidity-induced phytohormone abscisic acid (ABA) is the major regulator of active stomatal closure responses in angiosperms. Whether the ABA signalling pathway for stomatal closure functions similarly in older land plant groups, such as lycophytes and ferns, is still unclear: some studies find no stomatal ABA response in ferns, others find that ABA response is present or triggered by specific environmental conditions. Here we analysed steady-state gas-exchange, stomatal density and stomatal response to exogenously applied ABA in nine fern species grown from spores under controlled growth conditions. We find that ABA responses in ferns are species-specific: stomata in four out of nine species closed in response to ABA. The ABA-sensitive species mostly had slow responses of low magnitude, suggesting reduced ABA-sensitivity of ABA signalling pathway in ferns. Species with larger stomatal conductance tended to close stomata in response to ABA, whereas a relatively strong response of ~35% was also found in Cyrtomium falcatum, a fern with low stomatal conductance. Our results show that ferns constitute a diverse group with varying degree of stomatal ABA-sensitivity. Further characterisation of ABA signalling pathway components in diverse fern species is needed to understand the genetic basis for the variable ABA-sensitivity in ferns.