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bioRxiv · 10.1101/2023.03.07.531515

ORC1 binds to cis-transcribed RNAs for efficient activation of replication origins

Abstract

Cells must coordinate the activation of thousands of replication origins dispersed throughout their genome. Active transcription is known to favor the formation of mammalian origins, although the role that RNA plays in this process remains unclear. We show that the ORC1 subunit of the human Origin Recognition Complex interacts with RNAs transcribed from genes with origins in their transcription start sites (TSSs), displaying a positive correlation between RNA binding and origin activity. RNA depletion, or the use of ORC1 RNA-binding mutant, result in inefficient activation of proximal origins, linked to impaired ORC1 chromatin release. ORC1 RNA binding activity resides in its intrinsically disordered region, involved in intra- and inter-molecular interactions, regulation by phosphorylation, and phase-separation. We show that RNA binding favors ORC1 chromatin release, by regulating its phosphorylation and subsequent degradation. We propose that fluctuating concentrations of RNA during the cell cycle may play a sequential role in controlling origins through interaction with this flexible region of ORC1. Our results unveil a novel non-coding function of RNA as a dynamic component of the chromatin, orchestrating the activation of replication origins. One sentence summaryThe human origin recognition complex subunit 1 ORC1, binds to RNAs transcribed from genes with origins of replication at the TSS, which is required for optimal origin activation.

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BibTeXRIS

Mas, A. M., Goni, E., Ruiz de los Mozos, I., Arcas, A., Statello, L., Gonzalez, J., Blazquez, L., Lee, W. T. C., Gupta, D., Sejas, A., Hoshina, S., Armaos, A., Tartaglia, G. G., Waga, S., Ule, J., Rothenberg, E., Gomez, M., Huarte, M.. 2023-03-07. ORC1 binds to cis-transcribed RNAs for efficient activation of replication origins. https://doi.org/10.1101/2023.03.07.531515

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