Recent developments in the production of 2D- and 3D colon and stomach adenocarcinomas primary cell models

Tokarchuck, I. M.; Mushii, O.; Dreus, A.; Koziaruk, A. A.; Shapochka, D. O.; Ryzhak, O. A.; Venhryk, S. O.; Golovko, I. S.; Ryabuhin, S. V.; Hanopolskyi, A. I.; Judd, D. B.; Volochnyuk, D. M.

doi:10.1101/2023.02.26.529716

bioRxiv · 10.1101/2023.02.26.529716

Recent developments in the production of 2D- and 3D colon and stomach adenocarcinomas primary cell models

Tokarchuck, I. M.·Mushii, O.·Dreus, A.·Koziaruk, A. A.·Shapochka, D. O.·Ryzhak, O. A.·Venhryk, S. O.·Golovko, I. S.·Ryabuhin, S. V.·Hanopolskyi, A. I.·Judd, D. B.·Volochnyuk, D. M.

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Abstract

Gastric and colorectal cancer models are essential for the advancement of precision medicine discovery and development. 2D attached monolayer, spheroid and organoid approaches have all been used in the formation of biobanks containing primary patient-derived cells. Here, we report an assessment of those procedures for a panel of nine patient-derived adenocarcinoma samples, along with the most applicable method for the bio-banking of these cell types. A live cell biobank of tumour specimens would facilitate drug discovery laboratories to evaluate drugs on the population of cell cultures, prior to the clinical phase.

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Tokarchuck, I. M., Mushii, O., Dreus, A., Koziaruk, A. A., Shapochka, D. O., Ryzhak, O. A., Venhryk, S. O., Golovko, I. S., Ryabuhin, S. V., Hanopolskyi, A. I., Judd, D. B., Volochnyuk, D. M.. 2023-02-26. Recent developments in the production of 2D- and 3D colon and stomach adenocarcinomas primary cell models. https://doi.org/10.1101/2023.02.26.529716

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