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bioRxiv · 10.1101/2023.02.22.529480

Image-based Flow Simulation of Platelet Aggregates under Different Shear Rates

Abstract

Hemodynamics is crucial for the activation and aggregation of platelets in response to flow-induced shear. In this paper, a novel image-based computational model simulating blood flow through and around platelet aggregates is presented. The microstructure of aggregates was captured by two different modalities of microscopy images of in vitro whole blood perfusion experiments in microfluidic chambers coated with collagen. One set of images captured the geometry of the aggregate outline, while the other employed platelet labelling to infer the internal density. The platelet aggregates were modelled as a porous medium, the permeability of which was calculated with the Kozeny-Carman equation. The computational model was subsequently applied to study hemodynamics inside and around the platelet aggregates. The blood flow velocity, shear stress and kinetic force exerted on the aggregates were investigated and compared under 800 s-1, 1600 s-1 and 4000 s-1 wall shear rates. The advection-diffusion balance of agonist transport inside the platelet aggregates was also evaluated by local Peclet number. The findings show that the transport of agonists is not only affected by the shear rate but also significantly influenced by the microstructure of the aggregates. Moreover, large kinetic forces were found at the transition zone from shell to core of the aggregates, which could contribute to identifying the boundary between the shell and the core. The shear rate and the rate of elongation flow were investigated as well. The results imply that the emerging shapes of aggregates are highly correlated to the shear rate and the rate of elongation. The framework provides a way to incorporate the internal microstructure of the aggregates into the computational model and yields a better understanding of the hemodynamics and physiology of platelet aggregates, hence laying the foundation for predicting aggregation and deformation under different flow conditions. Author summaryThe initial step in the formation of an arterial thrombus is the rapid aggregation of the tiny blood particles called platelets. This process significantly influences the formation and structure of the resulting thrombi. The mechanical properties of the aggregates depend on their microstructure, which in turn is dictated by their interaction with the flow during formation. However, due to currently existing technological limitations, it is not possible to measure these interactions in sufficient detail experimentally. In this paper, an image-based computational model is proposed based on two different modalities of experimental images, that can complement the experiments and give detailed information on hemodynamics during the aggregation. The image sets are captured from whole blood perfused microfluidic chambers coated with collagen. One modality of images captured the shape of the aggregate outline with high contrast, while the other employed platelet labeling to infer the internal density. The platelet aggregates are considered as porous media in the simulations, informed by the images. This framework incorporates the internal microstructure of the aggregates into the computational model and yields a better understanding of the hemodynamics and physiology of platelet aggregates, hence laying the foundation for predicting aggregation and deformation under different flow conditions.

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Hao, Y., Zavodszky, G., Tersteeg, C., Barzegari, M., Hoekstra, A. G.. 2023-02-22. Image-based Flow Simulation of Platelet Aggregates under Different Shear Rates. https://doi.org/10.1101/2023.02.22.529480

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