Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets

Akopian, A.; Ruparel, S.; Hovhannisyan, A. H.; Lindquist, K.; Tram, M.; Perez, D.; Mecklenburg, J.; Salmon, A.; Merlo, J.; Corey, T.

doi:10.1101/2023.02.10.528062

bioRxiv · 10.1101/2023.02.10.528062

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets

Akopian, A.·Ruparel, S.·Hovhannisyan, A. H.·Lindquist, K.·Tram, M.·Perez, D.·Mecklenburg, J.·Salmon, A.·Merlo, J.·Corey, T.

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Abstract

Myogenous temporomandibular disorders (TMDM) is associated with an increased responsiveness of nerves innervating the masseter (MM), temporal (TM), medial pterygoid (MPM) and lateral pterygoid muscles (LPM). This study aimed to examine sensory nerve types innervating MM, TM and LPM of adult non-human primate - common marmosets. Sensory nerves are localized in specific regions of these muscles. Pgp9.5, marker for all nerves, and NFH, a marker for A-fibers, showed that masticatory muscles were predominantly innervated with A-fibers. The proportion of C- to A-fibers was highest in LPM, and minimal (6-8%) in MM. All C-fibers (pgp9.5+/NFH-) observed in masticatory muscles were peptidergic (CGRP+) and lacked mrgprD, trpV1 and CHRNA3, a silent nociceptive marker. All fibers in masticatory muscles were labeled with GFAP+, a myelin sheath marker. There were substantially more peptidergic A-fibers (CGRP+/NFH+) in TM and LPM compared to MM. Almost all A-fibers in MM expressed trkC, with some of them having trkB and parvalbumin. In contrast, a lesser number of TM and LPM nerves expressed trkC, and lacked trkB. Tyrosine hydroxylase antibodies, which did not label TG, highlighted sympathetic fibers around blood vessels of the masticatory muscles. Overall, masticatory muscle types of marmosets have distinct and different innervation patterns.

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Akopian, A., Ruparel, S., Hovhannisyan, A. H., Lindquist, K., Tram, M., Perez, D., Mecklenburg, J., Salmon, A., Merlo, J., Corey, T.. 2023-02-12. Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets. https://doi.org/10.1101/2023.02.10.528062

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