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bioRxiv · 10.1101/2023.02.07.527497

GAUT10 is required for Arabidopsis root cell differentiation and elongation

Dash, L.·Swaminathan, S.·Simura, J.·Montes, C.·Solanki, N.·Mejia, L.·Ljung, K.·Zabotina, O.·Kelley, D. R.
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Abstract

O_LICell wall properties of the root apical meristem (RAM) are poorly understood compared to the elongation and maturation zones of the developing root. GAUT10 is a pectin biosynthesizing enzyme that is post-transcriptionally regulated by auxin that influences Arabidopsis RAM size in a sucrose-dependent manner. C_LIO_LIUsing live-cell microscopy, we have determined the short root phenotype of the gaut10 loss of function allele is due to a reduction in both RAM cell number and epidermal cell elongation. In addition, the absence of GAUT10 leads to a reduction in lateral root cap and epidermal cell marker line expression, indicating root cell differentiation defects in this mutant. C_LIO_LIGAUT10 is required for normal pectin and hemicellulose composition in primary Arabidopsis roots. Loss of GAUT10 leads to a reduction in galacturonic acid and xylose in primary cell walls and alters the presence of rhamnogalacturonan (RG) I and homogalacturonan (HG) polymers in the root. C_LIO_LIAuxin mediated gene expression and metabolism is altered in gaut10 roots, suggesting that cell wall composition may influence auxin pathways. C_LI

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Dash, L., Swaminathan, S., Simura, J., Montes, C., Solanki, N., Mejia, L., Ljung, K., Zabotina, O., Kelley, D. R.. 2023-02-07. GAUT10 is required for Arabidopsis root cell differentiation and elongation. https://doi.org/10.1101/2023.02.07.527497

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