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bioRxiv · 10.1101/2023.02.05.527216

Bioinformatic analysis of the spike protein cleavage sites of coronaviruses in the mammalian order Eulipotyphla

Guo, Q.·Choi, A.·Millet, J.·Whittaker, G.
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Abstract

The mammalian order Eulipotyphla, including hedgehogs and shrews, represent a poorly understood reservoir of coronaviruses with zoonotic potential. Here, we carried out a bioinformatic analyses of these viruses based on the viral spike protein--to illustrate the complexity of coronavirus evolutionary history and the diversity of viruses from these host species, with a focus on the presence of possible furin cleavage sites within the spike protein. We found no evidence for cleavage by furin itself; however, certain strains of Wencheng Sm Shrew coronavirus were shown to have a predicted cleavage site for other member of the proprotein convertases, which are furin family members-- suggesting their spillover potential. As the expanding urbanization and the trade of small mammals in the wet markets enhance the wildlife-human interactions, this may increase pathogen spillover risks. Therefore, we should implement broad wild animal surveillance and be vigilant of contact with these small wild mammals in light of one-health perspectives.

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Guo, Q., Choi, A., Millet, J., Whittaker, G.. 2023-02-06. Bioinformatic analysis of the spike protein cleavage sites of coronaviruses in the mammalian order Eulipotyphla. https://doi.org/10.1101/2023.02.05.527216

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