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bioRxiv · 10.1101/2023.01.27.525999

ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics

Yong, W. K.·Rane, G.·Zaal Anuar, N.·Shao, X.·Goh, C. Y.·Khanchandani, V.·Kuan, V. L. S.·Jeitany, M.·Koeffler, P.·Deng, L.-W.·Sanda, T.·Kappei, D.
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Abstract

Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combined ChIP with label-free quantitative (LFQ) mass spectrometry (ChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-driven locus-specific enrichment.

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Yong, W. K., Rane, G., Zaal Anuar, N., Shao, X., Goh, C. Y., Khanchandani, V., Kuan, V. L. S., Jeitany, M., Koeffler, P., Deng, L.-W., Sanda, T., Kappei, D.. 2023-01-28. ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics. https://doi.org/10.1101/2023.01.27.525999

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