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bioRxiv · 10.1101/2023.01.26.525195

Spatial Multimodal Analysis of Transcriptomes and Metabolomes in Tissues

Vicari, M.·Mirzazadeh, R.·Nilsson, A.·Shariatgorji, R.·Bjarterot, P.·Larsson, L.·Lee, H.·Nilsson, M.·Foyer, J.·Ekvall, M.·Czarnewski, P.·Zhang, X.·Svenningsson, P.·Andren, P.·Lundeberg, J.
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Abstract

We present a spatial omics approach that merges and expands the capabilities of independently performed in situ assays on a single tissue section. Our spatial multimodal analysis combines histology, mass spectrometry imaging, and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular weight metabolites across tissue regions. We demonstrate the potential of our method using murine and human brain samples in the context of dopamine and Parkinsons disease.

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Vicari, M., Mirzazadeh, R., Nilsson, A., Shariatgorji, R., Bjarterot, P., Larsson, L., Lee, H., Nilsson, M., Foyer, J., Ekvall, M., Czarnewski, P., Zhang, X., Svenningsson, P., Andren, P., Lundeberg, J.. 2023-01-27. Spatial Multimodal Analysis of Transcriptomes and Metabolomes in Tissues. https://doi.org/10.1101/2023.01.26.525195

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