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bioRxiv · 10.1101/2023.01.24.525305

Bacillus subtilis RadA/Sms-mediated nascent lagging-strand un-winding at stalled or reversed forks is a two-step process: RadA/Sms assists RecA nucleation, and RecA loads RadA/Sms

Torres, R.·Carrasco, B.·Alonso, J. C.
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Abstract

Replication fork rescue requires Bacillus subtilis RecA, its negative (SsbA) and positive (RecO) mediators, and fork-processing (RadA/Sms). To understand how they work to promote fork remodeling, reconstituted branched replication intermediates were used. We show that RadA/Sms (or its variant RadA/Sms C13A) binds to the 5'-tail of a reversed fork with longer nascent lagging-strand and unwinds it in the 5'[->]3' direction, but RecA and its mediators limit unwinding. RadA/Sms cannot unwind a reversed fork with a longer nascent leading-strand, or a gapped stalled fork, but RecA interacts with and activates unwinding. Here, the molecular mechanism by which RadA/Sms, in concert with RecA, in a two-step reaction, unwinds the nascent lagging-strand of reversed or stalled forks is unveiled. First, RadA/Sms, as a mediator, contributes to SsbA displacement from the forks, and nucleates RecA onto single-stranded DNA. Then, RecA, as a loader, interacts with and recruits RadA/Sms onto the nascent lagging strand of these DNA substrates to unwind them. Within this process, RecA limits RadA/Sms self-assembly to control fork processing, and RadA/Sms prevents RecA from provoking unnecessary recombination.

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Torres, R., Carrasco, B., Alonso, J. C.. 2023-01-24. Bacillus subtilis RadA/Sms-mediated nascent lagging-strand un-winding at stalled or reversed forks is a two-step process: RadA/Sms assists RecA nucleation, and RecA loads RadA/Sms. https://doi.org/10.1101/2023.01.24.525305

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