sLASER and PRESS Perform Similarly at Revealing Metabolite-Age Correlations

Hui, S. C. N.; Gong, T.; Zoellner, H. J.; Hupfeld, K. E.; Gudmundson, A.; Murali-Manohar, S.; Davies-Jenkins, C.; Song, Y.; Chen, Y.; Oeltzschner, G.; Wang, G.; Edden, R. A. E.

doi:10.1101/2023.01.18.524597

bioRxiv · 10.1101/2023.01.18.524597

sLASER and PRESS Perform Similarly at Revealing Metabolite-Age Correlations

Hui, S. C. N.·Gong, T.·Zoellner, H. J.·Hupfeld, K. E.·Gudmundson, A.·Murali-Manohar, S.·Davies-Jenkins, C.·Song, Y.·Chen, Y.·Oeltzschner, G.·Wang, G.·Edden, R. A. E.

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Abstract

PurposeTo compare the respective ability of PRESS and sLASER to reveal biological relationships, using age as a validation covariate. MethodsMRS data were acquired from 102 healthy volunteers using PRESS and sLASER in centrum semiovale (CSO) and posterior cingulate cortex (PCC) regions. Acquisition parameters included TR/TE 2000/30 ms; 96 transients; 2048 datapoints sampled at 2 kHz. Spectra were analyzed using Osprey. Signal-to-noise ratio (SNR), full-width-half-maximum linewidth of tCr, and metabolite concentrations were extracted. A linear model was used to compare SNR and linewidth. Paired t-tests were used to assess differences in metabolite measurements between PRESS and sLASER. Correlations were used to evaluate the relationship between PRESS and sLASER metabolite estimates, as well as the strength of each metabolite-age relationship. Coefficients of variation were calculated to assess inter-subject variability in each metabolite measurement. ResultsSNR and linewidth were significantly higher (p<0.05) for sLASER than PRESS. Paired t-tests showed significant differences between PRESS and sLASER in most metabolite measurements. Metabolite measures were significantly correlated (p<0.05) for most metabolites between the two methods except GABA, Gln and Lac in CSO and GSH, Lac and NAAG in PCC. Metabolite-age relationships were consistently identified using both PRESS and sLASER. Similar CVs were observed for most metabolites. ConclusionThe study results suggest strong agreement between PRESS and sLASER in identifying relationships between brain metabolites and age in CSO and PCC data acquired at 3T. sLASER is technically desirable due to the reduced chemical shift displacement artifact; however, PRESS performed similarly in good brain regions at clinical field strength.

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Hui, S. C. N., Gong, T., Zoellner, H. J., Hupfeld, K. E., Gudmundson, A., Murali-Manohar, S., Davies-Jenkins, C., Song, Y., Chen, Y., Oeltzschner, G., Wang, G., Edden, R. A. E.. 2023-01-20. sLASER and PRESS Perform Similarly at Revealing Metabolite-Age Correlations. https://doi.org/10.1101/2023.01.18.524597

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