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bioRxiv · 10.1101/2023.01.16.524304

Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin is Conferred by Translational Regulation

Bashir, S.·Sun, X.-M.·Zhao, Y.·Martinez-Illescas, N. G.·Gallego-Lopez, I.·Guerrero Negron, L.·Keifenheim, D.·Karadimitriou, T.·Tran, T.·Pickering, M.·Marguerat, S.·Rhind, N.
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Abstract

The major fission yeast cyclin, Cdc13, has been shown to increase in concentration in correlation with cell size, and has been proposed to thereby regulate cell size at division. However, the mechanism of its cell-size regulation has been unknown. Here, we show that Cdc13 is regulated post-transcriptionally. Its transcript is not expressed in a size-dependent manner, rather a size-dependent concentration of protein is expressed from a size-independent concentration of mRNA. Moreover, we show that the expression of Cdc13 is, in fact, size dependent, as opposed to simply size-correlated due to time-dependent expression. We identify a 20-amino-acid motif, which includes the APC D-box degron, as necessary and sufficient for size-dependent expression, which allowed us to construct a size-independent allele of cdc13. Using this allele, we show that size-dependent expression of Cdc13 is not required for size control in fission yeast cells.

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Bashir, S., Sun, X.-M., Zhao, Y., Martinez-Illescas, N. G., Gallego-Lopez, I., Guerrero Negron, L., Keifenheim, D., Karadimitriou, T., Tran, T., Pickering, M., Marguerat, S., Rhind, N.. 2023-01-16. Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin is Conferred by Translational Regulation. https://doi.org/10.1101/2023.01.16.524304

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