Nutrient niche partitioning between tropical legumes and grasses strengthens under elevated CO2

Churchill, A. C.; Zhang, H.; Kim, G. w.; Catunda, K. L. M.; Anderson, I. C.; Isbell, F. I.; Moore, B.; Pendall, E.; Plett, J. M.; Powell, J.; Power, S. A.

doi:10.1101/2023.01.16.524162

bioRxiv · 10.1101/2023.01.16.524162

Nutrient niche partitioning between tropical legumes and grasses strengthens under elevated CO2

Churchill, A. C.·Zhang, H.·Kim, G. w.·Catunda, K. L. M.·Anderson, I. C.·Isbell, F. I.·Moore, B.·Pendall, E.·Plett, J. M.·Powell, J.·Power, S. A.

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Abstract

O_LIPlant community biodiversity can be maintained, at least partially, by shifts in species interactions between facilitation and competition for resources as environmental conditions change. These interactions also drive ecosystem functioning, including productivity, and can promote over-yielding-an ecosystem service prioritized in agro-ecosystems, such as pastures, that occurs when multiple species together are more productive than the component species alone. Importantly, species interactions that can result in over-yielding may shift in response to rising CO2 concentrations and changes in resource availability, and the consequences these shifts have on production is uncertain especially in the context of tropical mixed-species grasslands. C_LIO_LIWe examined the relative performance of two species pairs of tropical pasture grasses and legumes growing in monoculture and mixtures in a glasshouse experiment manipulating CO2. We investigated how over-yielding can arise from nitrogen (N) niche partitioning and biotic facilitation using stable isotopes to differentiate soil N from biological N fixation (BNF) within N acquisition into aboveground biomass for these two-species mixtures. C_LIO_LIWe found that N niche partitioning in species-level use of soil N vs. BNF drove species interactions in mixtures. Importantly partitioning and overyielding were generally reduced under elevated CO2. However, this finding was mixture-dependent based on biomass of dominant species in mixtures and the strength of selection effects for the dominant species. C_LIO_LIThis study demonstrates that rising atmospheric CO2 may alter niche partitioning between co-occurring species, with negative implications for the over-yielding benefits predicted for legume-grass mixtures in working landscapes with tropical species. Furthermore, these changes in inter-species interactions may have consequences for grassland composition that are not yet considered in larger-scale projections for impacts of climate change and species distributions. C_LI Graphical abstract(Image by H. Zhang): Among our tropical pasture species we found that grasses (dotted lines) grown in monoculture rely fully on soil nitrogen (N), while legumes (solid lines) grown in monoculture relied approximately equally on soil N and biological nitrogen fixation (BNF) to meet N requirements. When grown with tropical grasses, however, legumes shifted to rely more strongly on BNF, indicative of niche partitioning and decreased competition for soil nutrients with grasses. This separation of niche space was weakened under elevated CO2 conditions, ultimately reducing legume production. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=120 SRC="FIGDIR/small/524162v3_ufig1.gif" ALT="Figure 1"> View larger version (44K): org.highwire.dtl.DTLVardef@70de5corg.highwire.dtl.DTLVardef@1685f0org.highwire.dtl.DTLVardef@b3662corg.highwire.dtl.DTLVardef@1f911a3_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Churchill, A. C., Zhang, H., Kim, G. w., Catunda, K. L. M., Anderson, I. C., Isbell, F. I., Moore, B., Pendall, E., Plett, J. M., Powell, J., Power, S. A.. 2023-01-19. Nutrient niche partitioning between tropical legumes and grasses strengthens under elevated CO2. https://doi.org/10.1101/2023.01.16.524162

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