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bioRxiv · 10.1101/2023.01.15.524169

Label-free quantitative proteomics analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii

Xie, H.·Xu, C.·Zhao, G.·Dong, H.·Dai, L.·Xu, H.·Zhang, L.·Sun, H.·Wang, Q.·Zhang, J.·Yin, K.
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Abstract

Toxoplasma gondii (T. gondii) is an opportunistic parasite that can infect the central nervous system (CNS), causing severe toxoplasmosis and behavioral cognitive impairment. Mortality is high in immunocompromised individuals with toxoplasmosis, most commonly due to reactivation of infection in the CNS. There are still no effective vaccines and drugs for the prevention and treatment of toxoplasmosis. There are five developmental stages for T. gondii to complete life cycle, of which the tachyzoite and bradyzoite stages are the key to the acute and chronic infection. In this study, to better understanding of how T. gondii interacts with the host CNS at different stages of infection, we constructed acute and chronic infection models of T. gondii in astrocytes, and used label-free proteomics to detect the proteome changes before and after infection, respectively. A total of 4676 proteins were identified, among which 163 differentially expressed proteins (DEPs) (fold change[≥]1.5 or [≤]0.67 and p-value[≤]0.05) including 109 up-regulated proteins and 54 down-regulated proteins in C8-TA vs C8 group, and 719 DEPs including 495 up-regulated proteins and 224 down-regulated proteins in C8-BR vs C8-TA group. After T. gondii tachyzoites infected astrocytes, DEPs were enriched in immune-related biological processes to promote the formation of bradyzoites and maintain the balance of T. gondii, CNS and brain. After T. gondii bradyzoites infected astrocytes, the DEPs up-regulated the hosts glucose metabolism, and some up-regulated DEPs were closely related to neurodegenerative diseases. These findings not only provide new insights into the psychiatric pathogenesis of T. gondii, but also provide potential targets for the treatment of acute and chronic Toxoplasmosis.

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Xie, H., Xu, C., Zhao, G., Dong, H., Dai, L., Xu, H., Zhang, L., Sun, H., Wang, Q., Zhang, J., Yin, K.. 2023-01-19. Label-free quantitative proteomics analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii. https://doi.org/10.1101/2023.01.15.524169

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