Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Bosch, C.; Lindenau, J.; Pacureanu, A.; Peddie, C. J.; Majkut, M.; Douglas, A. C.; Carzaniga, R.; Rack, A.; Collinson, L.; Schaefer, A.; Stegmann, H.

doi:10.1101/2023.01.10.523473

bioRxiv · 10.1101/2023.01.10.523473

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Bosch, C.·Lindenau, J.·Pacureanu, A.·Peddie, C. J.·Majkut, M.·Douglas, A. C.·Carzaniga, R.·Rack, A.·Collinson, L.·Schaefer, A.·Stegmann, H.

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Abstract

Correlative multimodal imaging is a useful approach to investigate complex structural relations in life sciences across multiple scales. For these experiments, sample preparation workflows that are compatible with multiple imaging techniques must be established. In one such implementation, a fluorescently-labelled region of interest in a biological soft tissue sample can be imaged with light microscopy before staining the specimen with heavy metals, enabling follow-up higher resolution structural imaging at the targeted location, bringing context where it is required. Alternatively, or in addition to fluorescence imaging, other microscopy methods such as synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT) or serial blockface scanning electron microscopy (SBF-SEM) might also be applied. When combining imaging techniques across scales, it is common that a volumetric region of interest (ROI) needs to be carved from the total sample volume before high resolution imaging with a subsequent technique can be performed. In these situations, the overall success of the correlative workflow depends on the precise targeting of the ROI and the trimming of the sample down to a suitable dimension and geometry for downstream imaging. Here we showcase the utility of a novel femtosecond laser device to prepare microscopic samples (1) of an optimised geometry for synchrotron X-ray microscopy as well as (2) for subsequent volume electron microscopy applications, embedded in a wider correlative multimodal imaging workflow (Fig. 1). O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=172 SRC="FIGDIR/small/523473v1_fig1.gif" ALT="Figure 1"> View larger version (42K): org.highwire.dtl.DTLVardef@1c72db6org.highwire.dtl.DTLVardef@18a234forg.highwire.dtl.DTLVardef@142bd0org.highwire.dtl.DTLVardef@4894f0_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOFigure 1.C_FLOATNO Correlative microscopy workflows incorporating samples processed using a femtosecond laser. C_FIG

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Bosch, C., Lindenau, J., Pacureanu, A., Peddie, C. J., Majkut, M., Douglas, A. C., Carzaniga, R., Rack, A., Collinson, L., Schaefer, A., Stegmann, H.. 2023-01-12. Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences. https://doi.org/10.1101/2023.01.10.523473

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