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bioRxiv · 10.1101/2023.01.09.523325

Polyploid yeast are dependent on elevated levels of Mps1 for successful chromosome segregation

Abstract

Tumor cell lines with elevated chromosome numbers frequently exhibit elevated expression of Mps1. These tumors are also dependent on high Mps1 activity for their survival. Mps1 is a conserved kinase involved in controlling aspects of chromosome segregation in mitosis and meiosis. The mechanistic explanation for the Mps1-addiction of aneuploid cells is unknown. To address this question, we explored Mps1-dependence in yeast cells with increased sets of chromosomes. These experiments revealed that in yeast, increasing ploidy leads to delays and failures in orienting chromosomes on the mitotic spindle. Yeast cells with elevated numbers of chromosomes proved vulnerable to reductions of Mps1 activity. Cells with reduced Mps1 activity exhibit an extended prometaphase with longer spindles and delays in orienting the chromosomes. One known role of Mps1 is in recruiting Bub1 to the kinetochore in meiosis. We found that the Mps1-addiction of polyploid yeast cells is due in part to its role in Bub1 recruitment. Together, the experiments presented here demonstrate that increased ploidy renders cells more dependent on Mps1 for orienting chromosomes on the spindle. The phenomenon described here may be relevant in understanding why high-ploidy cancer cells exhibit elevated reliance on Mps1 expression for successful chromosome segregation. AUTHOR SUMMARYLosing or gaining chromosomes during cell division leads to aneuploidy (an abnormal number of chromosomes) and can contribute to cancer and other diseases. Indeed, most cells in solid tumors carry abnormally elevated numbers of chromosomes. Mps1 is a regulator of the machinery that distributes chromosomes to daughter cells. In tumors with elevated chromosome numbers, the expression of Mps1 is often also elevated. In some aneuploid tumor cell lines these elevated Mps1 levels have been shown to be critical for tumor survival. To determine how cells with higher ploidy become dependent on Mps1, we explored Mps1-dependence in yeast cells with increased numbers of chromosomes. We report that yeast cells with elevated chromosome number are sensitive to reductions Mps1 expression. In cells with high ploidy and reduced levels of Mps1, the progression of the cell cycle is delayed and the ability of the cells to properly orient and segregate their chromosomes on the spindle is greatly reduced.

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BibTeXRIS

Meyer, R. E., Sartin, A., Gish, M., Harsha, J., Wilkie, E., Haworth, D., LaVictorie, R., Alberola, I., Chuong, H. H., Gorbsky, G. J., Dawson, D. S.. 2023-01-10. Polyploid yeast are dependent on elevated levels of Mps1 for successful chromosome segregation. https://doi.org/10.1101/2023.01.09.523325

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