Metabolite T1 relaxation times differ across the adult lifespan

Murali-Manohar, S.; Gudmundson, A. T.; Hupfeld, K. E.; Zöllner, H. J.; Hui, S. C. N.; Song, Y.; Davies-Jenkins, C. W.; Gong, T.; Wang, G.; Oeltzschner, G.; Edden, R. A. E.

doi:10.1101/2023.01.06.522927

bioRxiv · 10.1101/2023.01.06.522927

Metabolite T1 relaxation times differ across the adult lifespan

Murali-Manohar, S.·Gudmundson, A. T.·Hupfeld, K. E.·Zöllner, H. J.·Hui, S. C. N.·Song, Y.·Davies-Jenkins, C. W.·Gong, T.·Wang, G.·Oeltzschner, G.·Edden, R. A. E.

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Abstract

PurposeTo investigate the age-dependence of metabolite T1 relaxation times at 3T in both gray- and white-matter-rich voxels. MethodsThis manuscript analyzes publicly available metabolite and metabolite-nulled (single inversion recovery TI = 600 ms) spectra acquired at 3T using PRESS localization. Voxels were placed in posterior cingulate cortex and centrum semiovale in 102 healthy volunteers across 5 decades of life (20s to 60s). All spectra were analyzed in Osprey v2.4.0. To estimate T1 relaxation times for tNAA2.0 and tCr3.0, the ratio of modeled metabolite residual amplitudes in the metabolite-nulled spectrum to the full metabolite signal was calculated using the single inversion recovery signal equation. Correlations between T1 and subject age were evaluated. ResultsSpearman correlations revealed that estimated T1 relaxation times of tNAA2.0 (rs = -0.43; p < 0.001) and tCr3.0 (rs = -0.23; p = 0.021) decreased significantly with age in white-matter-rich CSO, and less steeply (and not significantly) for tNAA2.0 (rs = -0.15; p = 0.136) and tCr3.0 (rs = -0.10; p = 0.319) in gray-matter-rich PCC. ConclusionThe analysis harnessed a large publicly available cross-sectional dataset to test an important hypothesis, that metabolite T1 relaxation times change with age. This preliminary study stresses the importance of further work to measure age-normed metabolite T1 relaxation times for accurate quantification of metabolite levels in studies of aging.

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Murali-Manohar, S., Gudmundson, A. T., Hupfeld, K. E., Zöllner, H. J., Hui, S. C. N., Song, Y., Davies-Jenkins, C. W., Gong, T., Wang, G., Oeltzschner, G., Edden, R. A. E.. 2023-01-08. Metabolite T1 relaxation times differ across the adult lifespan. https://doi.org/10.1101/2023.01.06.522927

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