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bioRxiv · 10.1101/2022.12.31.521896

Regionalized protein localization domains in the hair cell kinocilium

Erickson, T.·Biggers, W. P.·Williams, K.·Venuto, A.
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Abstract

Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by "hair"-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of actin-filled stereocilia, the hair bundle features a single, non-motile, true cilium called the kinocilium. The kinocilium plays important roles in bundle development and the mechanics of sensory detection. To understand more about kinocilial development and structure, we performed a transcriptomic analysis of zebrafish hair cells looking for cilia-associated genes that have yet to be characterized in hair cells. Here, we focus on three such genes - ankef1a, odf3l2a, and saxo2 - because the human or mouse orthologs are either associated with sensorineural hearing loss or are located near uncharacterized deafness loci. We made transgenic fish that express fluorescently tagged versions of their proteins, demonstrating their localization to the kinocilia of zebrafish hair cells. Furthermore, we find that Ankef1a, Odf3l2a, and Saxo2 exhibit distinct localization patterns along the length of the kinocilium and within the cell body. Lastly, we report a novel overexpression phenotype for Saxo2. Overall, these results suggest that the hair cell kinocilium is regionalized along its proximal-distal axis and set the groundwork to understand more about the roles of these kinocilial proteins in hair cells.

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Erickson, T., Biggers, W. P., Williams, K., Venuto, A.. 2023-01-02. Regionalized protein localization domains in the hair cell kinocilium. https://doi.org/10.1101/2022.12.31.521896

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