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bioRxiv · 10.1101/2022.12.30.522321

Expanded tRNA methyltransferase family member TRMT9B regulates synaptic growth and function

Hogan, C. A.·Gratz, S. J.·Dumouchel, J. M.·Delgado, A.·Lentini, J. M.·Madhwani, K. R.·Thakur, R. S.·Fu, D.·O'Connor-Giles, K. M.
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Abstract

Nervous system function relies on the formation and function of synaptic connections between neurons. Through a genetic screen in Drosophila for new conserved synaptic genes, we identified CG42261/Fid/ TRMT9B as a negative regulator of synaptogenesis. TRMT9B has been studied for its role as a tumor suppressor in multiple carcinomas and is one of two metazoan homologs of yeast tRNA methyltransferase 9 (Trm9), which methylates tRNA wobble uridines. Members of the expanded family of tRNA methyltransferases are increasingly being associated with neurological disorders and new biochemical functions. Interestingly, whereas Trm9 homolog ALKBH8/CG17807 is ubiquitously expressed, we find that TRMT9B is enriched in the nervous system, including at synapses. However, in the absence of animal models the role of TRMT9B in the nervous system has remained unknown. Here, we generated null alleles of TRMT9B and ALKBH8, and through liquid chromatography-mass spectrometry find that ALKBH8 is responsible for canonical tRNA wobble uridine methylation under basal conditions. In the nervous system, we find that TRMT9B negatively regulates synaptogenesis through a methyltransferase-dependent mechanism in agreement with our modeling studies. Finally, we find that neurotransmitter release is impaired in TRMT9B mutants. Our findings reveal a role for TRMT9B in regulating synapse formation and function, and highlight the importance of the expanded family of tRNA methyltransferases in the nervous system.

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Hogan, C. A., Gratz, S. J., Dumouchel, J. M., Delgado, A., Lentini, J. M., Madhwani, K. R., Thakur, R. S., Fu, D., O'Connor-Giles, K. M.. 2022-12-31. Expanded tRNA methyltransferase family member TRMT9B regulates synaptic growth and function. https://doi.org/10.1101/2022.12.30.522321

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