Bovine blastocyst like structures derived from stem cell cultures

Pinzon-Arteaga, C. A.; Wang, Y.; Wei, Y.; Li, L.; Ribeiro Orsi, A. E.; Scatolin, G.; Liu, L.; Sakurai, M.; Ye, J.; Yu, L.; Li, B.; Jiang, Z.; Wu, J.

doi:10.1101/2022.12.20.521301

bioRxiv · 10.1101/2022.12.20.521301

Bovine blastocyst like structures derived from stem cell cultures

Pinzon-Arteaga, C. A.·Wang, Y.·Wei, Y.·Li, L.·Ribeiro Orsi, A. E.·Scatolin, G.·Liu, L.·Sakurai, M.·Ye, J.·Yu, L.·Li, B.·Jiang, Z.·Wu, J.

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Abstract

Understanding blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. We developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via the assembly of trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, and represent an accessible in vitro model for studying bovine embryogenesis.

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Pinzon-Arteaga, C. A., Wang, Y., Wei, Y., Li, L., Ribeiro Orsi, A. E., Scatolin, G., Liu, L., Sakurai, M., Ye, J., Yu, L., Li, B., Jiang, Z., Wu, J.. 2022-12-21. Bovine blastocyst like structures derived from stem cell cultures. https://doi.org/10.1101/2022.12.20.521301

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