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bioRxiv · 10.1101/2022.12.20.521175

A Jacob/nsmf gene knockout does not protect against hypoxia- and NMDA-induced neuronal cell death

Monteiro Gomes, G.·Bar, J.·Karpova, A.·Kreutz, M.
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Abstract

Jacob is a synapto-nuclear messenger protein that encodes and transduces the origin of synaptic and extrasynaptic NMDA receptor signals to the nucleus. The protein assembles a signalosome that differs in case of synaptic or extrasynaptic NMDAR activation. Following nuclear import Jacob docks these signalosomes to the transcription factor CREB. We have recently shown that amyloid-beta and extrasynaptic NMDAR activation triggers the translocation of a Jacob signalosome that results in inactivation of the transcription factor CREB, a phenomenon termed Jacob-induced CREB shut-off (JaCS). JaCS contributes to early Alzheimers disease pathology and a gene knockout of nsmf, the gene encoding Jacob, protects against amyloid pathology. Given that extrasynaptic activity is also involved in acute excitotoxicity, like in stroke, we asked whether nsmf gene knockout will also protect against acute insults, like oxygen and glucose deprivation and excitotoxic NMDA stimulation. Here we show that organotypic hippocampal slices from wild-type and nsmf-/- mice display similar degrees of degeneration when exposed to either oxygen glucose deprivation or 50 {micro}M NMDA incubation. This lack of neuroprotection indicates that JaCS is mainly relevant in conditions of low level chronic extrasynaptic NMDAR activation that results in cellular degeneration induced by alterations in gene transcription.

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Monteiro Gomes, G., Bar, J., Karpova, A., Kreutz, M.. 2022-12-21. A Jacob/nsmf gene knockout does not protect against hypoxia- and NMDA-induced neuronal cell death. https://doi.org/10.1101/2022.12.20.521175

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