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bioRxiv · 10.1101/2022.12.08.519681

New Aequorea fluorescent proteins for cell-free bioengineering

Deich, C.·Gaut, N. J.·Sato, W.·Engelhart, A. E.·Adamala, K. P.
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Abstract

Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms. O_FIG O_LINKSMALLFIG WIDTH=144 HEIGHT=200 SRC="FIGDIR/small/519681v1_ufig1.gif" ALT="Figure 1"> View larger version (41K): org.highwire.dtl.DTLVardef@3a3100org.highwire.dtl.DTLVardef@672233org.highwire.dtl.DTLVardef@f65f32org.highwire.dtl.DTLVardef@e40aaf_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Deich, C., Gaut, N. J., Sato, W., Engelhart, A. E., Adamala, K. P.. 2022-12-09. New Aequorea fluorescent proteins for cell-free bioengineering. https://doi.org/10.1101/2022.12.08.519681

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