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bioRxiv · 10.1101/2022.12.06.519343

Pulse-chase experiments reveal dynamics of RNA binding protein Exuperantia in Drosophila melanogaster egg chambers

Vieira, D. V.·Carlota, R. R.·de-Carvalho, J.·Telley, I. A.
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Abstract

In cells, mRNA can be associated with various proteins, forming ribonucleoprotein complexes (RNPs) which take part in spatiotemporal control of translation. In the Drosophila melanogaster developing egg chamber, a set of RNPs is transported from the nurse cells to the oocyte and targeted selectively to specific cellular locations. This mRNA sorting process leads to the final oocyte polarization pre-defining the body axes of the future embryo. However, how mRNA is encoded for selection and directed transport is mechanistically not well understood. A master mRNA involved in body axes formation is bicoid, which localizes anterolaterally and is essential for head and thorax definition of the embryo. A protein that was identified essential for bicoid anterior localization is Exuperantia (Exu). Here, we use a live imaging-based pulse-chase approach, which reveals selective transport dynamics of Exu from nurse cells to the oocyte during mid to late-stage oogenesis.

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Vieira, D. V., Carlota, R. R., de-Carvalho, J., Telley, I. A.. 2022-12-06. Pulse-chase experiments reveal dynamics of RNA binding protein Exuperantia in Drosophila melanogaster egg chambers. https://doi.org/10.1101/2022.12.06.519343

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