Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Nemergut, M.; Pluskal, D.; Horackova, J.; Sustrova, T.; Tulis, J.; Baatallah, R.; Gagnot, G.; Novakova, V.; Majerova, M.; Marques, S. M.; Toul, M.; Damborsky, J.; Bednar, D.; Prokop, Z.; Janin, Y. L.; Marek, M.

doi:10.1101/2022.12.05.519101

bioRxiv · 10.1101/2022.12.05.519101

Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Nemergut, M.·Pluskal, D.·Horackova, J.·Sustrova, T.·Tulis, J.·Baatallah, R.·Gagnot, G.·Novakova, V.·Majerova, M.·Marques, S. M.·Toul, M.·Damborsky, J.·Bednar, D.·Prokop, Z.·Janin, Y. L.·Marek, M.

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Abstract

NanoLuc, a superior {beta}-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques were combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an allosteric site shaped on the enzyme surface. Structurally, binding to the allosteric site prevents simultaneous binding to the catalytic site, and vice versa, through concerted conformational changes. We demonstrate that restructuration of the allosteric site can boost the luminescent reaction in the remote active site. Mechanistically, an intra-barrel arginine coordinates the imidazopyrazinone component of luciferin, which reacts with O2 via a radical charge-transfer mechanism, and then it also protonates the resulting excited amide product to form a light-emitting neutral species. Concomitantly, an aspartate, supported by two tyrosines, fine-tunes the blue color emitter to secure a high emission intensity. This information is critical to engineering the next-generation of ultrasensitive bioluminescent reporters.

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Nemergut, M., Pluskal, D., Horackova, J., Sustrova, T., Tulis, J., Baatallah, R., Gagnot, G., Novakova, V., Majerova, M., Marques, S. M., Toul, M., Damborsky, J., Bednar, D., Prokop, Z., Janin, Y. L., Marek, M.. 2022-12-05. Illuminating the mechanism and allosteric behavior of NanoLuc luciferase. https://doi.org/10.1101/2022.12.05.519101

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