Cis-regulatory evolution of the potassium channel gene kcnj13 during pigment pattern diversification in Danio fish

Podobnik, M.; Singh, A. P.; Fu, Z.; Dooley, C. M.; Frohnhoefer, H. G.; Firlej, M.; Elhabashy, H.; Weyand, S.; Weir, J.; Lu, J.; Nuesslein-Volhard, C.; Irion, U.

doi:10.1101/2022.12.05.519077

bioRxiv · 10.1101/2022.12.05.519077

Cis-regulatory evolution of the potassium channel gene kcnj13 during pigment pattern diversification in Danio fish

Podobnik, M.·Singh, A. P.·Fu, Z.·Dooley, C. M.·Frohnhoefer, H. G.·Firlej, M.·Elhabashy, H.·Weyand, S.·Weir, J.·Lu, J.·Nuesslein-Volhard, C.·Irion, U.

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Abstract

Teleost fish of the genus Danio are excellent models to study the genetic and cellular bases of pigment pattern variation in vertebrates. The two sister species Danio rerio and Danio aesculapii show divergent patterns of horizontal stripes and vertical bars that are partly caused by the evolution of the potassium channel gene kcnj13. In D. rerio, kcnj13 is required in melanophores for interactions with xanthophores and iridophores, which cause location-specific pigment cell shapes and thereby influence colour pattern and contrast. Here, we show that cis-regulatory rather than protein coding changes underlie kcnj13 evolution between the two species. D. aesculapii express lower kcnj13 levels and exhibit low-contrast patterns similar to D. rerio mutants. Our results suggest that homotypic and heterotypic interactions between the pigment cells and their shapes diverged between species by quantitative changes in kcnj13 expression during pigment pattern diversification.

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Podobnik, M., Singh, A. P., Fu, Z., Dooley, C. M., Frohnhoefer, H. G., Firlej, M., Elhabashy, H., Weyand, S., Weir, J., Lu, J., Nuesslein-Volhard, C., Irion, U.. 2022-12-05. Cis-regulatory evolution of the potassium channel gene kcnj13 during pigment pattern diversification in Danio fish. https://doi.org/10.1101/2022.12.05.519077

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