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bioRxiv · 10.1101/2022.11.30.518278

Different low-complexity regions of SFPQ play distinct roles in the formation of biomolecular condensates

Abstract

Demixing of proteins and nucleic acids into condensed liquid phases is rapidly emerging as a ubiquitous mechanism governing the organisation of molecules within the cell. Long disordered low complexity regions (LCRs) are a common feature of proteins that form biomolecular condensates. RNA-binding proteins with prion-like composition have been highlighted as drivers of liquid demixing to form condensates such as nucleoli, paraspeckles and stress granules. Splicing factor proline- and glutamine-rich (SFPQ) is an RNA- and DNA-binding protein essential for DNA repair and paraspeckle formation. Here, we show that the shorter C-terminal LCR of SFPQ is the main region responsible for the condensation of SFPQ in vitro and in the cell. In contrast, we find that, unexpectedly, the longer N-terminal prion-like LCR of SFPQ attenuates condensation, suggesting a more regulatory role in preventing aberrant condensate formation in the cell. Our data add nuance to the emerging understanding of biomolecular condensate formation, by providing the first example of a common multifunctional nucleic acid-binding protein with an extensive prion-like region that serves to regulate rather than drive condensate formation. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=120 SRC="FIGDIR/small/518278v2_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@ed464dorg.highwire.dtl.DTLVardef@1a26fdcorg.highwire.dtl.DTLVardef@15debecorg.highwire.dtl.DTLVardef@12bd4ae_HPS_FORMAT_FIGEXP M_FIG C_FIG

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BibTeXRIS

Marshall, A. C., Cummins, J., Kobelke, S., Zhu, T., Widagdo, J., Anggono, V., Hyman, A., Fox, A. H., Bond, C. S., Lee, M.. 2022-11-30. Different low-complexity regions of SFPQ play distinct roles in the formation of biomolecular condensates. https://doi.org/10.1101/2022.11.30.518278

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