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bioRxiv · 10.1101/2022.11.22.517543

IFNγ drives neuroinflammation and demyelination in a mouse model of multiple system atrophy

Gallups, N. J.·Childers, G. M.·Webster, J. M.·Yang, Y.-T.·Zane, A.·Mudium, N.·Manfredsson, F.·Kordower, J.·Harms, A. S.
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Abstract

Multiple system atrophy (MSA) is a rare and fatal synucleinopathy characterized by insoluble alpha-synuclein (-syn) cytoplasmic inclusions located within oligodendroglia. Neuroinflammation, demyelination, and neurodegeneration are correlated with areas of GCI pathology, however it is not known what specifically drives disease pathogenesis. Recently in a mouse model of MSA, CD4+ T cells have been shown to drive neuroinflammation and demyelination, however the mechanism by which this occurs also remains unclear. In this study we use genetic and pharmacological approaches in a novel model of MSA to show that the pro-inflammatory cytokine interferon gamma (IFN{gamma}) drives neuroinflammation and demyelination. Furthermore, using an IFN{gamma} reporter mouse, we found that infiltrating CD4+ T cells were the primary producers of IFN{gamma} in response to -syn overexpression in oligodendrocytes. Results from these studies indicate that IFN{gamma} expression in CD4 T cells drives -syn-mediated neuroinflammation and demyelination, and strategies to target IFN{gamma} expression may be a potential disease modifying therapeutic strategy for MSA.

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Gallups, N. J., Childers, G. M., Webster, J. M., Yang, Y.-T., Zane, A., Mudium, N., Manfredsson, F., Kordower, J., Harms, A. S.. 2022-11-23. IFNγ drives neuroinflammation and demyelination in a mouse model of multiple system atrophy. https://doi.org/10.1101/2022.11.22.517543

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