Comprehensive glycoprofiling of oral tumours associates N-glycosylation with lymph node metastasis and patient survival

Moretto Carnielli, C.; Melo de Lima Morais, T.; Malta de Sa Patroni, F.; Prado Ribeiro, A. C.; Brandao, T. B.; Sobroza, E.; Luongo Matos, L.; Kowalski, L. P.; Paes Leme, A.; Kawahara, R.; Thaysen-Andersen, M.

doi:10.1101/2022.11.21.517331

bioRxiv · 10.1101/2022.11.21.517331

Comprehensive glycoprofiling of oral tumours associates N-glycosylation with lymph node metastasis and patient survival

Moretto Carnielli, C.·Melo de Lima Morais, T.·Malta de Sa Patroni, F.·Prado Ribeiro, A. C.·Brandao, T. B.·Sobroza, E.·Luongo Matos, L.·Kowalski, L. P.·Paes Leme, A.·Kawahara, R.·Thaysen-Andersen, M.

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Abstract

While altered protein glycosylation is regarded a trait of oral squamous cell carcinoma (OSCC), its heterogeneous glycoproteome and dynamics with disease progression remain unmapped. To this end, we here employ an integrated multi-omics approach comprising unbiased and quantitative glycomics and glycoproteomics applied to a valuable cohort of resected tumour tissues from OSCC patients with (n = 19) and without (n = 12) lymph node metastasis. While all tumour tissues displayed uniform N-glycome profiles suggesting relatively stable global N-glycosylation during lymph node metastasis, glycoproteomics and advanced correlation analysis notably uncovered altered site-specific N-glycosylation and previously unknown associations with several key clinicopathological features. Importantly, focused analyses of the multi-omics data unveiled two N-glycans and three N-glycopeptides that were closely associated with patient survival. This study provides novel insight into the complex OSCC tissue N-glycoproteome forming an important resource to further explore the underpinning disease mechanisms and uncover new prognostic glyco-markers for OSCC. TeaserDeep survey of the dynamic landscape of complex sugars in oral tumours paves a way for new prognostic disease markers.

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Moretto Carnielli, C., Melo de Lima Morais, T., Malta de Sa Patroni, F., Prado Ribeiro, A. C., Brandao, T. B., Sobroza, E., Luongo Matos, L., Kowalski, L. P., Paes Leme, A., Kawahara, R., Thaysen-Andersen, M.. 2022-11-22. Comprehensive glycoprofiling of oral tumours associates N-glycosylation with lymph node metastasis and patient survival. https://doi.org/10.1101/2022.11.21.517331

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