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bioRxiv · 10.1101/2022.11.19.517202

Split selectable marker mediated gene stacking in plants

Yuan, G.·Lu, H.·De, K.·Hassan, M. M.·Wellington, M.·Tuskan, G. A.·Yang, X.
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Abstract

Plant synthetic biology and genetic engineering depend on the controlled expression of transgenes of interest, which relies heavily on the use of Agrobacterium-mediated transformation. Here we establish a novel split selectable marker system using protein splicing elements called "inteins" for Agrobacterium-mediated co-transformation in plants. This method enables robust co-transformation in Arabidopsis and poplar, providing a novel strategy for the simultaneous insertion of multiple genes into both herbaceous and woody plants.

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Yuan, G., Lu, H., De, K., Hassan, M. M., Wellington, M., Tuskan, G. A., Yang, X.. 2022-11-20. Split selectable marker mediated gene stacking in plants. https://doi.org/10.1101/2022.11.19.517202

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