Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM)

Louvel, V.; Haase, R.; Mercey, O.; Laporte, M. H.; Soldati-Favre, D.; Hamel, V.; Guichard, P.

doi:10.1101/2022.11.14.516383

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bioRxiv · 10.1101/2022.11.14.516383

Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM)

Louvel, V.·Haase, R.·Mercey, O.·Laporte, M. H.·Soldati-Favre, D.·Hamel, V.·Guichard, P.

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Abstract

Expansion microscopy is an approach of super-resolution fluorescence microscopy that does not yet achieve the precision of nanoscopy techniques such as single-molecule light microscopy (SMLM). Here, we developed an iterative ultrastructure expansion microscopy approach (iU-ExM), which now matches the SMLM resolution as demonstrated using standard references such as the nuclear pores. Applicable to both cells and tissues, iU-ExM allows a broad research community to access high precision super-resolution microscopy.

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Louvel, V., Haase, R., Mercey, O., Laporte, M. H., Soldati-Favre, D., Hamel, V., Guichard, P.. 2022-11-15. Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM). https://doi.org/10.1101/2022.11.14.516383

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