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bioRxiv · 10.1101/2022.11.12.516247

Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

Hauke, L.·Isbaner, S.·Ghosh, A.·Guido, I.·Turco, L.·Chizhik, A. I.·Gregor, I.·Karedla, N.·Rehfeldt, F.·Enderlein, J.
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Abstract

Metal-Induced Energy Transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of a microscope. Although its capability in numerous biological and biophysical studies has been demonstrated, its implementation for live-cell imaging with fluorescent proteins is still lacking. Here, we present its applicability and capabilities for live-cell imaging with fluorescent proteins in diverse cell types (adult human stem cells, human osteo-sarcoma cells, and Dictyostelium discoideum cells), and with various fluorescent proteins (GFP, mScarlet, RFP, YPet). We show that MIET imaging achieves nanometer axial mapping of living cellular and sub-cellular components across multiple timescales, from a few milliseconds to hours, with negligible phototoxic effects.

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Hauke, L., Isbaner, S., Ghosh, A., Guido, I., Turco, L., Chizhik, A. I., Gregor, I., Karedla, N., Rehfeldt, F., Enderlein, J.. 2022-11-13. Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins. https://doi.org/10.1101/2022.11.12.516247

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