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bioRxiv · 10.1101/2022.11.11.516163

Mapping phosphorylation post-translational modifications along single peptides with nanopores

Nova, I. C.·Ritmejeris, J.·Brinkerhoff, H.·Koenig, T. J. R.·Gundlach, J. H.·Dekker, C.
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Abstract

Current methods to detect post-translational modifications (PTMs) of proteins, such as phosphate groups, cannot measure single molecules and often cannot differentiate between closely spaced phosphorylation sites. Using a nanopore sequencing approach, we here report detection of PTMs at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants. We reliably discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.

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Nova, I. C., Ritmejeris, J., Brinkerhoff, H., Koenig, T. J. R., Gundlach, J. H., Dekker, C.. 2022-11-12. Mapping phosphorylation post-translational modifications along single peptides with nanopores. https://doi.org/10.1101/2022.11.11.516163

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