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bioRxiv · 10.1101/2022.11.08.515584

Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Bootz-Maoz, H.·Simon, A.·Del Mare-Roumani, S.·Bennet, Y.·Zheng, D.·Amidror, S.·Elinav, E.·Yissachar, N.
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Abstract

The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex-vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

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Bootz-Maoz, H., Simon, A., Del Mare-Roumani, S., Bennet, Y., Zheng, D., Amidror, S., Elinav, E., Yissachar, N.. 2022-11-08. Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics. https://doi.org/10.1101/2022.11.08.515584

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