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bioRxiv · 10.1101/2022.11.07.515222

Mosaic chromosomal alterations in blood across ancestries via whole-genome sequencing

Abstract

Mosaic mutations in blood are common with increasing age and are prognostic markers for cancer, cardiovascular dysfunction and other diseases. This group of acquired mutations include megabase-scale mosaic chromosomal alterations (mCAs). These large mutations have mainly been surveyed using SNP array data from individuals of European (EA) or Japanese genetic ancestry. To gain a better understanding of mCA rates and associated risk factors in genetically diverse populations, we surveyed whole genome sequencing data from 67,390 individuals, including 20,132 individuals of African ancestry (AA), and 7,608 of Hispanic ancestry (HA) with deep (30X) whole genome sequencing data from the NHLBI Trans Omics for Precision Medicine (TOPMed) program. We adapted an existing mCA calling algorithm for application to WGS data, and observed higher sensitivity with WGS data, compared with array-based data, in uncovering mCAs at low mutant cell fractions. As in previous reports, we observed a strong association with age and a non-uniform distribution of mCAs across the genome. The presence of autosomal (but not chromosome X) mCAs was associated with an increased risk of both lymphoid and myeloid malignancies. After adjusting for age, we found that individuals of European ancestry have the highest rates of autosomal mCAs, mirroring the higher rate of leukemia in this group. Our analysis also uncovered higher rates of chromosome X mCAs in AA and HA compared to EA, again after adjusting for age. Germline variants in ATM and MPL showed strong associations with mCAs in cis, including ancestry specific variants. And rare variant gene-burden analysis confirmed the association of putatively protein altering variants in ATM and MPL with mCAs in cis. Individual rare variants in DCPS, ADM17, PPP1R16B, and TET2 were all associated with autosomal mCAs and rare variants in OR4C16 were associated with chromosome X mCAs in females. There was significant enrichment of co-occurrence of CHIP mutations and mCAs both altering cancer associated genes TET2, DNMT3A, JAK2, CUX1, and TP53. Overall, our study demonstrates that rates of mCAs differ across populations and that rare inherited germline variants are strongly associated with mCAs across genetically diverse populations. These results strongly motivate further studies of mCAs in under-represented populations to better understand the causes and consequences of this class of somatic variation.

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BibTeXRIS

Auer, P. L., Jakubek, Y. A., Zhou, Y., Stilp, A. M., Bacon, J., Wong, J., Mitchell, B., Lewis, J., Boerwinkle, E., Loos, R. J. F., Preuss, M., Barnes, K., Pankratz, N., Fornage, M., Redline, S., Psaty, B. M., Bis, J. C., Silverman, E., Cho, M., Yun, J., Ramachandran, V., Mathias, R. A., Taub, M., Peyser, P. A., Smith, J. A., Kardia, S., Arnett, D., North, K. E., Raffield, L. M., Carson, A., Doyle, M., Rich, S., Rotter, J., Guo, X., Cox, N., Mack, T., Chasman, D., Machiela, M., Ozcan, Z., Conomos, M., Li, Y., Desai, P., Bick, A., Reiner, A. P., Scheet, P.. 2022-11-08. Mosaic chromosomal alterations in blood across ancestries via whole-genome sequencing. https://doi.org/10.1101/2022.11.07.515222

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