END-TO-END CLASSIFICATION OF CELL-CYCLE STAGES WITH CENTER-CELL FOCUS TRACKER USING RECURRENT NEURAL NETWORKS

Abstract

Cell division, or mitosis, guarantees the accurate inheritance of the genomic information kept in the cell nucleus. Malfunctions in this process cause a threat to the health and life of the organism, including cancer and other manifold diseases. It is therefore crucial to study in detail the cell-cycle in general and mitosis in particular. Consequently, a large number of manual and semi-automated time-lapse microscopy image analyses of mitosis have been carried out in recent years. In this paper, we propose a method for automatic detection of cell-cycle stages using a recurrent neural network (RNN). An end-to-end model with center-cell focus tracker loss, and classification loss is trained. The evaluation was conducted on two time-series datasets, with 6-stages and 3-stages of cell splitting labeled. The frame-to-frame accuracy was calculated and precision, recall, and F1-Score were measured for each cell-cycle stage. We also visualized the learned feature space. Image reconstruction from the center-cell focus module was performed which shows that the network was able to focus on the center-cell and classify it simultaneously. Our experiments validate the superior performance of the proposed network compared to a classifier baseline.