R-loop editing by DNA cytosine deaminase APOBEC3B determines the activity of estrogen receptor enhancers

Zhang, C.; LU, Y.-j.; Chen, B.; Bai, Z.; Hervieu, A.; Licciardello, M. P.; Wang, M.; Mitsopoulos, K.; Al-Lazikani, B. P.; Tortorici, M.; Rossanese, O.; Workman, P.; Clarke, P. A.

doi:10.1101/2022.10.21.513235

bioRxiv · 10.1101/2022.10.21.513235

R-loop editing by DNA cytosine deaminase APOBEC3B determines the activity of estrogen receptor enhancers

Zhang, C.·LU, Y.-j.·Chen, B.·Bai, Z.·Hervieu, A.·Licciardello, M. P.·Wang, M.·Mitsopoulos, K.·Al-Lazikani, B. P.·Tortorici, M.·Rossanese, O.·Workman, P.·Clarke, P. A.

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Abstract

Estrogen receptor (ER) activation results in the formation of DNA double strand breaks (DSB), which promote genomic instability and tumour heterogeneity in ER-positive breast cancers. The single-stranded DNA (ssDNA) cytosine deaminase APOBEC3B (A3B) regulates ER activity by inducing DSB at ER enhancers. To delineate how A3B recognises its substrates and unveil the underlying mechanism leading to the formation of ER-induced DSB, we sampled A3B-mediated deamination sites using whole genome sequencing in a human breast cancer cell model lacking base excision repair function. Our genome-wide analysis revealed that C>U conversions carried out by A3B in R-loop structures are processed into DSB in the vicinity of ER promoters or enhancers. A mechanism which required both the processing of A3B-editing sites and R-loops by distinct DNA damage repair mechanisms. In addition, using BioID-enabled mass-spectroscopy proteomics, we identified TDRD3 as a key A3B-binding partner directing the activity of A3B to ER-induced R-loops. This study suggests a function for A3B in sustaining tumour evolution as an adaptive response at the transcriptional and epigenetic level and supports A3B as a promising target to control ER activity in cancer.

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Zhang, C., LU, Y.-j., Chen, B., Bai, Z., Hervieu, A., Licciardello, M. P., Wang, M., Mitsopoulos, K., Al-Lazikani, B. P., Tortorici, M., Rossanese, O., Workman, P., Clarke, P. A.. 2022-10-22. R-loop editing by DNA cytosine deaminase APOBEC3B determines the activity of estrogen receptor enhancers. https://doi.org/10.1101/2022.10.21.513235

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