The Western Lake Erie Culture Collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome

Yancey, C. E.; Kiledal, E. A.; Denef, V. J.; Errera, R. M.; Evans, J. T.; Hart, L.; Isailovic, D.; James, W.; Kharbush, J. J.; Kimbrel, J. A.; Li, W.; Mayali, X.; Nitschky, H.; Polik, C.; Powers, M. A.; Premathilaka, S. H.; Rappuhn, N.; Reitz, L. A.; Rivera, S. R.; Zwiers, C. C.; Dick, G. J.

doi:10.1101/2022.10.21.513177

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bioRxiv · 10.1101/2022.10.21.513177

The Western Lake Erie Culture Collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome

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Abstract

Cyanobacteria harmful algal blooms (cyanoHABs) dominated by Microcystis spp. have significant public health and economic implications in freshwater bodies around the world. These blooms are capable of producing a variety of cyanotoxins, including microcystins, that affect fishing and tourism industries, human and environmental health, and access to drinking water. In this study, we isolated and sequenced the genomes of 21 unialgal Microcystis cultures collected from western Lake Erie between 2017-2019. While some cultures isolated in different years have a high degree of genetic similarity (Average Nucleotide Identity >99%), genomic data shows that these cultures also represent much of the breadth of known Microcystis diversity in natural populations. Only 5 isolates contained all the genes required for microcystin synthesis while 2 isolates contained a previously described partial mcy operon. Microcystin production within cultures was also assessed using Enzyme-Linked Immunosorbent Assay (ELISA) and supported genomic results with high concentrations (up to 900 g L-1) in cultures with complete mcy operons and no or low toxin detected otherwise. These xenic cultures also contained a substantial diversity of bacteria associated with Microcystis, which has become increasingly recognized as an essential component of cyanoHAB community dynamics. These results highlight the genomic diversity among Microcystis strains and associated bacteria in Lake Erie, and their potential impacts on bloom development, toxin production, and toxin degradation. This collection significantly increases the availability of environmentally relevant Microcystis strains from temperate North America, which is changing rapidly due to climate change. HighlightsO_LITwenty one xenic Microcystis cultures were isolated from western Lake Erie and capture the diversity of Microcystis strains observed in natural populations as well as their associated bacteria C_LIO_LIMicrocystis strains show variability in core and accessory gene content, and genetically similar strains produce varying concentrations and congeners of microcystins C_LIO_LIThis collection is a valuable resource for studying strain diversity and interactions between Microcystis and associated bacteria C_LIO_LIOur collection increases the availability of environmentally relevant strains from temperate North America, which is historically underrepresented in culture collections. C_LI

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Yancey, C. E., Kiledal, E. A., Denef, V. J., Errera, R. M., Evans, J. T., Hart, L., Isailovic, D., James, W., Kharbush, J. J., Kimbrel, J. A., Li, W., Mayali, X., Nitschky, H., Polik, C., Powers, M. A., Premathilaka, S. H., Rappuhn, N., Reitz, L. A., Rivera, S. R., Zwiers, C. C., Dick, G. J.. 2022-10-22. The Western Lake Erie Culture Collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome. https://doi.org/10.1101/2022.10.21.513177

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