Precisely patterned nanofibers made from extendable protein multiplexes

Bethel, N. P.; Borst, A. J.; Parmeggiani, F.; Bick, M. J.; Brunette, T.; Nguyen, H.; Kang, A.; Bera, A. K.; Carter, L.; Miranda, M. C.; Kibler, R.; Lamb, M.; Li, X.; Sankaran, B.; Baker, D.

doi:10.1101/2022.10.14.511843

bioRxiv · 10.1101/2022.10.14.511843

Precisely patterned nanofibers made from extendable protein multiplexes

Bethel, N. P.·Borst, A. J.·Parmeggiani, F.·Bick, M. J.·Brunette, T.·Nguyen, H.·Kang, A.·Bera, A. K.·Carter, L.·Miranda, M. C.·Kibler, R.·Lamb, M.·Li, X.·Sankaran, B.·Baker, D.

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Abstract

Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha helical coiled coils have such symmetric extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here, we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C2 to C8 and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibers can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibers can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.

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Bethel, N. P., Borst, A. J., Parmeggiani, F., Bick, M. J., Brunette, T., Nguyen, H., Kang, A., Bera, A. K., Carter, L., Miranda, M. C., Kibler, R., Lamb, M., Li, X., Sankaran, B., Baker, D.. 2022-10-14. Precisely patterned nanofibers made from extendable protein multiplexes. https://doi.org/10.1101/2022.10.14.511843

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