A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p

Jegers, C.; Franzmann, T. M.; Hübner, J.; Schneider, J.; Landerer, C.; Wittmann, S.; Toth-Petroczy, A.; Sprangers, R.; Hyman, A. A.; Alberti, S.

doi:10.1101/2022.10.11.511767

bioRxiv · 10.1101/2022.10.11.511767

A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p

Jegers, C.·Franzmann, T. M.·Hübner, J.·Schneider, J.·Landerer, C.·Wittmann, S.·Toth-Petroczy, A.·Sprangers, R.·Hyman, A. A.·Alberti, S.

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Abstract

Heat shock promotes the assembly of translation factors into condensates to facilitate the production of stress-protective proteins. How translation factors detect heat and assemble into condensates is not well understood. Here, we investigate heat-induced condensate assembly by the translation factor Ded1p from five different fungi, including Ded1p from Saccharomyces cerevisiae. Using targeted mutagenesis and in vitro reconstitution biochemistry, we find that heat-induced Ded1p assembly is driven by a conformational rearrangement of the folded helicase domain. This rearrangement determines the assembly temperature and the assembly of Ded1p into nanometer-sized particles, while the flanking intrinsically disordered regions engage in intermolecular interactions to promote assembly into micron-sized condensates. Using protein engineering, we identify six amino acid substitutions that determine most of the thermostability of a thermophilic Ded1p ortholog, thereby providing a molecular understanding underlying the adaptation of the Ded1p assembly temperature to the specific growth temperature of the species. We conclude that heat-induced assembly of Ded1p into translation factor condensates is regulated by a complex interplay of the structured domain and intrinsically disordered regions which is subject to evolutionary tuning.

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Jegers, C., Franzmann, T. M., Hübner, J., Schneider, J., Landerer, C., Wittmann, S., Toth-Petroczy, A., Sprangers, R., Hyman, A. A., Alberti, S.. 2022-10-12. A conserved and tunable mechanism for the temperature-controlled condensation of the translation factor Ded1p. https://doi.org/10.1101/2022.10.11.511767

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