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bioRxiv · 10.1101/2022.10.05.511002

Dissecting the cellular basis of age-related cognitive dysfunction: Chronic chemogenetic inhibition of somatostatin-positive interneurons in the dentate gyrus hilus induces memory impairments

Lyu, J.·Nagarajan, R.·Kambali, M.·Wang, M.·Rudolph, U.
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Abstract

The cellular basis of age-related impairments of hippocampal function is not fully understood. In order to evaluate the role of somatostatin-positive (Sst+) interneurons in the dentate gyrus hilus in this process, we chemogenetically inhibited Sst+ interneurons in the dentate gyrus (DG) hilus. Chronic chemogenetic inhibition (CCI) of these neurons resulted in increased c-Fos staining in the DG hilus, a decrease in the percentage of Gad67- and of Sst-expressing interneurons in the DG, and increased microglial activation in DG, CA3, and CA1. Total dendritic length and spine density were reduced in DG and CA1, suggesting reduced dendritic complexity. Behaviorally, the recognition index in an object recognition task and the percentage of spontaneous alternations in the Y maze were decreased, while in both initial and reversal learning in the Morris water maze the latencies to find the hidden platform were increased, suggesting cognitive dysfunction. Our findings establish a causal role for a reduced function of Sst+ interneurons in the DG hilus for cognitive decline and suggest that this reduced function may contribute to age-related impairments of learning and memory. Furthermore, our CCI mice may represent a cellularly defined model of hippocampal aging. SIGNIFICANCE STATEMENTNeuronal circuits and cellular processes underlying age-related cognitive dysfunction are not well understood. We observed that chronic chemogenetic inhibition of a defined cell type, somatostatin-positive interneurons in the dentate gyrus hilus, which have previously been found to be associated with cognitive dysfunction in aged rodents, is necessary and sufficient to elicit changes in expression of interneuronal markers, an increase in the activity of dentate gyrus granule cells, increased microglial activation across the entire hippocampus and an impairment of learning and memory-related tasks. Thus, inhibition of somatostatin-positive interneurons in the dentate gyrus hilus replicates changes that are also seen with normal aging, representing a novel cellularly defined animal model of hippocampal aging.

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Lyu, J., Nagarajan, R., Kambali, M., Wang, M., Rudolph, U.. 2022-10-07. Dissecting the cellular basis of age-related cognitive dysfunction: Chronic chemogenetic inhibition of somatostatin-positive interneurons in the dentate gyrus hilus induces memory impairments. https://doi.org/10.1101/2022.10.05.511002

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