Simultaneous multicolor fluorescence imaging using duplication-based PSF engineering

Van den Eynde, R.; Hertel, F.; Krajnik, B.; Hugelier, S.; Auer, A.; Schlichthaerle, T.; Jungmann, R.; Leutenegger, M.; Vandenberg, W.; Dedecker, P.

doi:10.1101/2022.10.04.510770

bioRxiv · 10.1101/2022.10.04.510770

Simultaneous multicolor fluorescence imaging using duplication-based PSF engineering

Van den Eynde, R.·Hertel, F.·Krajnik, B.·Hugelier, S.·Auer, A.·Schlichthaerle, T.·Jungmann, R.·Leutenegger, M.·Vandenberg, W.·Dedecker, P.

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Abstract

We present a way to encode more information in fluorescence imaging by splitting the emission into copies of the original point-spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the Circulator, an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.

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Van den Eynde, R., Hertel, F., Krajnik, B., Hugelier, S., Auer, A., Schlichthaerle, T., Jungmann, R., Leutenegger, M., Vandenberg, W., Dedecker, P.. 2022-10-05. Simultaneous multicolor fluorescence imaging using duplication-based PSF engineering. https://doi.org/10.1101/2022.10.04.510770

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