A Protocol for Single Nucleus RNAseq from Frozen Skeletal Muscle

Soule, T. G.; Pontifex, C. S.; Rosin, N.; Joel, M. M.; Lee, S.; Nguyen, M. D.; Chhibber, S.; Pfeffer, G.

doi:10.1101/2022.10.02.510482

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bioRxiv · 10.1101/2022.10.02.510482

A Protocol for Single Nucleus RNAseq from Frozen Skeletal Muscle

Soule, T. G.·Pontifex, C. S.·Rosin, N.·Joel, M. M.·Lee, S.·Nguyen, M. D.·Chhibber, S.·Pfeffer, G.

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Abstract

Single cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single nucleus RNA sequencing (snRNAseq) due to their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by snRNAseq. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease.

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Soule, T. G., Pontifex, C. S., Rosin, N., Joel, M. M., Lee, S., Nguyen, M. D., Chhibber, S., Pfeffer, G.. 2022-10-05. A Protocol for Single Nucleus RNAseq from Frozen Skeletal Muscle. https://doi.org/10.1101/2022.10.02.510482

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