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bioRxiv · 10.1101/2022.09.30.507111

Chromatin regulators in the TBX1 network confer risk for conotruncal heart defects in 22q11.2DS and sporadic congenital heart disease

Abstract

BackgroundCongenital heart disease (CHD) affecting the conotruncal region of the heart, occur in half of patients with 22q11.2 deletion syndrome. This syndrome is a rare disorder with relative genetic homogeneity that can facilitate identification of genetic modifiers. Haploinsufficiency of TBX1, mapped to the 22q11.2 region, encoding a T-box transcription factor, is one of the main genes for the etiology of the syndrome. We suggest that genetic modifiers of CHD in patients with 22q11.2 deletion syndrome may be in the TBX1 gene network. MethodsTo identify genetic modifiers of 22q11.2 deletion syndrome, we analyzed whole genome sequence of subjects with 22q11.2DS, of which 456 were cases with conotruncal heart defects and 537 were controls with normal cardiac structures. We retained the most damaging rare coding variants and examined 19 functional gene sets for association that were weighted upon expression of genes in cardiac progenitor cells in mouse embryos identified by RNA-sequencing. ResultsWe identified rare damaging coding variants in chromatin regulatory genes as modifiers of conotruncal heart defects in 22q11.2DS. Chromatin genes with recurrent damaging variants include EP400, KAT6A, KMT2C, KMT2D, NSD1, CHD7 and PHF21A. In total, we identified 37 chromatin regulatory genes, that may increase risk for conotruncal heart defects in 8.5% of 22q11.2 deletion syndrome cases. Many of these genes were identified as risk factors for sporadic CHD in the general population increasing the likelihood that these genes are medically important contributors for CHD. These genes are co-expressed in cardiac progenitor cells with TBX1, suggesting that they may be in the same genetic network. Some of the genes identified, such as KAT6A, KMT2C, CHD7 and EZH2, have been previously shown to genetically interact with TBX1 in mouse models, providing mechanistic validation of these genes found. ConclusionsOur findings indicate that disturbance of chromatin regulatory genes impact a TBX1 gene network serving as genetic modifiers of 22q11.2 deletion syndrome. Since some of these chromatin regulatory genes were found in individuals with sporadic CHD, we suggest that there are shared mechanisms involving the TBX1 gene network in the etiology of CHD.

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Zhao, Y., Wang, Y., Shi, L., McDonald-McGinn, D. M., Crowley, T. B., McGinn, D. E., Tran, O. T., Miller, D., Zackai, E., Johnston, H. R., Chow, E., Vorstman, J. A. S., Vingerhoets, C., van Amelsvoort, T., Gothelf, D., Swillen, A., Breckpot, J., Vermeesch, J. R., Eliez, S., Schneider, M., van den Bree, M. B., Owen, M. J., Kates, W., Repetto, G. M., Shashi, V., Schoch, K., Bearden, C. E., Digilio, M. C. E., Unolt, M., Putotto, C., Marino, B., Pontillo, M., Armando, M., Vicari, S., Angkustsiri, K., Campbell, L., Busa, T., Heine-Suner, D., Murphy, K. C., Murphy, D., Garcia-Minaur, S., Fernandez, L. 2022-10-03. Chromatin regulators in the TBX1 network confer risk for conotruncal heart defects in 22q11.2DS and sporadic congenital heart disease. https://doi.org/10.1101/2022.09.30.507111

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