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bioRxiv · 10.1101/2022.09.23.509237

Tunable dynamics in a multi-straintranscriptional pulse generator

Abstract

A major challenge in synthetic biology is the manipulation of engineered gene circuits toward a specified behavior. This challenge becomes more difficult as synthetic systems become more complex by incorporating additional genes or strains. Here we demonstrate that circuit dynamics can be tuned in synthetic consortia through the manipulation of strain fractions within the community. To do this, we constructed a microbial consortium comprised of three strains of engineered Escherichia coli that, when co-cultured, use homoserine lactone (HSL) mediated intercellular signaling to create a multi-strain incoherent type-1 feedforward loop (I1-FFL). Like naturally occurring I1-FFL motifs in gene networks, this engineered microbial consortium acts as a pulse generator of gene expression. We demonstrated that the amplitude of the pulse can be easily tuned by adjusting the relative population fractions of the strains. We created a mathematical model for the temporal dynamics of the microbial consortium and, using this model, identified population fractions that produced desired pulse characteristics. Our work demonstrates that intercellular gene circuits can be effectively tuned simply by adjusting the starting fractions of each strain type.

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BibTeXRIS

Zong, D. M., Sadeghpour, M., Molinari, S., Alnahhas, R. N., Hirning, A. J., Giannitsis, C., Ott, W., Josic, K., Bennett, M. R.. 2022-09-24. Tunable dynamics in a multi-straintranscriptional pulse generator. https://doi.org/10.1101/2022.09.23.509237

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