Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source: Turkish DeLight

Atalay, N.; Akcan, E. K.; Gul, M.; Ayan, E.; Destan, E.; Ertem, F. B.; Tokay, N.; Cakilkaya, B.; Nergiz, Z.; Karakadioglu, G.; Kepceoglu, A.; Yapici, I.; Tosun, B.; Baldir, N.; Yildirim, G.; Johnson, J. A.; Guven, O.; Shafiei, A.; Arslan, N. E.; Yilmaz, M.; Kulakman, C.; Paydos, S. S.; Cinal, Z. S.; Sabanoglu, K.; Pazarceviren, A.; Yilmaz, A.; Canbay, B.; Asci, B.; Kartal, E.; Tavli, S.; Caliseki, M.; Goc, G.; Mermer, A.; Yesilay, G.; Altuntas, S.; Tateishi, H.; Otsuka, M.; Fujita, M.; Tekin, S.; Ciftci, H.; Durdagi, S.; Dinler Doganay, G.; Karaca, E.; Kaplan Turkoz, B.; Kabasakal, B. V.; Kati

doi:10.1101/2022.09.03.506456

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bioRxiv · 10.1101/2022.09.03.506456

Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source: Turkish DeLight

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Abstract

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryo-crystallography has never been performed in Turkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, Turkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.

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Atalay, N., Akcan, E. K., Gul, M., Ayan, E., Destan, E., Ertem, F. B., Tokay, N., Cakilkaya, B., Nergiz, Z., Karakadioglu, G., Kepceoglu, A., Yapici, I., Tosun, B., Baldir, N., Yildirim, G., Johnson, J. A., Guven, O., Shafiei, A., Arslan, N. E., Yilmaz, M., Kulakman, C., Paydos, S. S., Cinal, Z. S., Sabanoglu, K., Pazarceviren, A., Yilmaz, A., Canbay, B., Asci, B., Kartal, E., Tavli, S., Caliseki, M., Goc, G., Mermer, A., Yesilay, G., Altuntas, S., Tateishi, H., Otsuka, M., Fujita, M., Tekin, S., Ciftci, H., Durdagi, S., Dinler Doganay, G., Karaca, E., Kaplan Turkoz, B., Kabasakal, B. V., Kati. 2022-09-05. Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source: Turkish DeLight. https://doi.org/10.1101/2022.09.03.506456

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