Interaction with C21ORF2 controls the cellular functions of the NEK1 kinase

Rouse, J.; Gregorczyk, M.; Pastore, G.; Lis, P.; Lange, S.; Lamoliatte, F.; Macartney, T.; Toth, R.; Brown, F.; Hastie, J.; Durocher, D.

doi:10.1101/2022.08.31.505651

bioRxiv · 10.1101/2022.08.31.505651

Interaction with C21ORF2 controls the cellular functions of the NEK1 kinase

Rouse, J.·Gregorczyk, M.·Pastore, G.·Lis, P.·Lange, S.·Lamoliatte, F.·Macartney, T.·Toth, R.·Brown, F.·Hastie, J.·Durocher, D.

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Abstract

NEK1 is a pleiotropic protein kinase implicated in mitosis, ciliogenesis and DNA repair but little is known about its regulation or targets. Its relevance for human health is underscored by the association of NEK1 mutations with human diseases including axial spondylometaphyseal dyplasia (SMD) and amyotrophic lateral sclerosis (ALS). Mutations in the C21ORF2 gene are associated with a similar pattern of human diseases, suggesting close functional links with NEK1. Here we report that in unperturbed, untransformed cells, endogenous NEK1 and C21ORF2 form a tight complex that does not appear to contain other proteins. A small acidic domain "CID: C21ORF2 interaction domain" at the C-terminus of NEK1 is necessary and sufficient to interact with C21ORF2, and pathogenic mutations in this region disrupt the complex. AlphaFold modelling predicts with high confidence an extended binding interface between a leucine-rich repeat (LRR) domain in the N-terminal half of C21ORF2 and a stretch of the NEK1-CID; mutating residues mediating electrostatic interactions within this interface disrupts the NEK1-C21ORF2 interaction. This model also explains why pathogenic mutations disrupt the complex. We go on to show that the kinase activity of NEK1 and its interaction with C21ORF2 is critical for NEK1 function in cells. These data reveal C21ORF2 as a regulatory subunit of NEK1, illuminating our understanding of how this kinase is regulated and NEK1-C21ORF2-associated diseases.

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Rouse, J., Gregorczyk, M., Pastore, G., Lis, P., Lange, S., Lamoliatte, F., Macartney, T., Toth, R., Brown, F., Hastie, J., Durocher, D.. 2022-08-31. Interaction with C21ORF2 controls the cellular functions of the NEK1 kinase. https://doi.org/10.1101/2022.08.31.505651

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