The Immunological Proteome Resource

Brenes, A. J.; Hukelmann, J. L.; Spinelli, L.; Howden, A. J. M.; Marchingo, J. M.; Sinclair, L. V.; Rollings, C.; James, O. J.; Phair, I. R.; Matthews, S. P.; Ross, S. H.; Arthur, S. C.; Swamy, M.; Finlay, D. K.; Lamond, A. I. I.; Cantrell, D. A.

doi:10.1101/2022.08.29.505666

bioRxiv · 10.1101/2022.08.29.505666

The Immunological Proteome Resource

Brenes, A. J.·Hukelmann, J. L.·Spinelli, L.·Howden, A. J. M.·Marchingo, J. M.·Sinclair, L. V.·Rollings, C.·James, O. J.·Phair, I. R.·Matthews, S. P.·Ross, S. H.·Arthur, S. C.·Swamy, M.·Finlay, D. K.·Lamond, A. I. I.·Cantrell, D. A.

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Abstract

The Immunological Proteome Resource (ImmPRes; http://immpres.co.uk/) is an open access public resource integrating proteomic data generated by large-scale mass-spectrometry analysis of murine hematopoietic populations. The initial focus is T lymphocytes and how their proteomes are shaped by immune activation, environment, and intracellular signalling pathways with an aim to expand it to B cells and innate immune cells. It is a multidisciplinary effort between immunology and mass spectrometry-based labs with the objective to help define an in-depth high-quality map of immune cell proteomes. Maintaining data reproducibility and integrity are a priority within the resource, thus there is an in-depth protocols section explaining in detail the sample processing and the mass spectrometry-based analysis. ImmPRes provides open access to proteomic datasets covering a wide range of murine leukocyte populations with analysis of copy numbers per cell of > 10,000 proteins, enabling new understanding of lymphocyte phenotypes. All data is accessible via a simple graphical interface that supports easy interrogation of the data and options to download data summaries and raw data files.

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Brenes, A. J., Hukelmann, J. L., Spinelli, L., Howden, A. J. M., Marchingo, J. M., Sinclair, L. V., Rollings, C., James, O. J., Phair, I. R., Matthews, S. P., Ross, S. H., Arthur, S. C., Swamy, M., Finlay, D. K., Lamond, A. I. I., Cantrell, D. A.. 2022-08-29. The Immunological Proteome Resource. https://doi.org/10.1101/2022.08.29.505666

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