Effects of stimulus timing on the acquisition of an olfactory working memory task in head-fixed mice

Abstract

Knowing what factors affect the acquisition of a behavioural task is central to understanding the mechanisms of learning and memory. It also has practical implications, as animal behavioural experiments used to probe cognitive functions often require long training durations. Delayed Match (or Non-Match)-to-Sample (DMS/DNMS) tasks are relatively complex tasks used to study working memory and sensory perception, but their use in the mouse remains hampered by the lengthy training involved. In this study, we assessed two aspects of stimulus timing on the acquisition of an olfactory DNMS task: how the sample-test odour delay durations and the reward timing affect the acquisition rate. We demonstrate that head-fixed mice learn to perform an olfactory DNMS task more quickly when the initial training uses a shorter sample-test odour delay without detectable loss of generalisability. Unexpectedly, we observed a slower task acquisition when the odour-reward interval was shorter. This effect was accompanied by a shortening of reaction times and more frequent sporadic licking. Analysis of this result using a drift-diffusion model indicated that a primary consequence of early reward delivery is a lower decision bound. Since an accurate performance with a lower decision bound requires greater discriminability in the sensory representations, this may underlie the slower learning rate with early reward arrival. Together, our results reflect the possible effects of stimulus timing on stimulus encoding and its consequence on the acquisition of a complex task.