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bioRxiv · 10.1101/2022.08.22.504802

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Rezaei, A.·Bahmani, H. R.·Mafakheri, S.·Farshad, A.·Nazari, P.
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Abstract

The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen collection was performed every 5 days from 5 bucks (10 ejaculates per buck). The ejaculates were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. Each semen sample was frozen using a standard protocol, and sperm function and antioxidative status were evaluated after thawing. Results indicated higher total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower DNA fragmentation and malondialdehyde in MT200+Tr than for all other groups except MT200; meanwhile, MT200 was also greater in these parameters than in the control group (P < 0.05). Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high membrane mitochondrial potential than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improves the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.

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Rezaei, A., Bahmani, H. R., Mafakheri, S., Farshad, A., Nazari, P.. 2022-08-22. Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen. https://doi.org/10.1101/2022.08.22.504802

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